Mechanism of KSHV Infection and Cellular Transformation: A Rover for Probing Cancer
Dr. Shou-Jiang Gao’s laboratory is interested in cancer viruses and their associated cancers with the current focus on Kaposi’s sarcoma-associated herpesvirus (KSHV) and AIDS-related malignancies. Dr. Gao’s lab uses a systems approach to dissect KSHV-cell interactions and delineate the mechanisms of KSHV infection and cellular transformation. He will specifically discuss a model of KSHV-induced cellular transformation of primary cells, which consists of transformed cells and matched primary cells that are usually not available in other cancer models, and how this model is used to delineate KSHV-induced oncogenesis. A comprehensive approach involving reverse genetics, expression profiling, epigenetics, epitranscriptomics, metabolomics, Crispr-Cas9 genome-wide screening and high throughput screening was used to identify potential cancer vulnerable genes and tumor suppressors of KSHV-induced cancers. Ongoing works are to examine whether these identified targets can also be extended to other types of cancer.