DesignSignatures: a tool for designing primers that yields amplicons with distinct signatures

Wright ES, Vetsigian KH. DesignSignatures: a tool for designing primers that yields amplicons with distinct signatures. Bioinformatics. 2016 May 15;32(10):1565-7. doi: 10.1093/bioinformatics/btw047. Epub 2016 Jan 23. PMID: 26803162.

For numerous experimental applications, PCR primers must be designed to efficiently amplify a set of homologous DNA sequences while giving rise to amplicons with maximally diverse signatures. We developed DesignSignatures to automate the process of designing primers for high-resolution melting (HRM), fragment length polymorphism (FLP) and sequencing experiments. The program also finds the best restriction enzyme to further diversify HRM or FLP signatures. This enables efficient comparison across many experimental designs in order to maximize signature diversity.

AVAILABILITY AND IMPLEMENTATION:

DesignSignatures is accessible as a web tool at www.DECIPHER.cee.wisc.edu, or as part of the DECIPHER open source software package for R available from BioConductor.

CONTACT:

kalin@discovery.wisc.edu

SUPPLEMENTARY INFORMATION:

Supplementary data are available at Bioinformatics online.

Publication Year: 
2016
Faculty Author: 
Publication Credits: 
Wright ES, Vetsigian KH
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